PpoR is a conserved unpaired LuxR solo of Pseudomonas putida which binds N-acyl homoserine lactones.

ABSTRACT:

Background

Only a small number of Pseudomonas putida strains possess the typical N-acyl homoserine lactone quorum sensing system (AHL QS) that consists of a modular LuxR family protein and its cognate LuxI homolog that produces the AHL signal. Moreover, AHL QS systems in P. putida strains are diverse in the type of AHLs they produce and the phenotypes that they regulate.

Results

We identified an unpaired LuxR solo (QS luxR homolog that occurs without the corresponding luxI homolog), which is highly conserved in both the AHL producing and non-AHL producing P. putida strains that we analyzed. In this study we report the cloning and functional characterization of this unpaired LuxR homolog designated PpoR. An AHL binding assay showed that PpoR protein binds to 3-oxo-C6-HSL. Stu

SUBMITTER: Subramoni S 

PROVIDER: S-EPMC2703642 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature