Biomarkers for early and late stage chronic allograft nephropathy by proteogenomic profiling of peripheral blood.

ABSTRACT:

Background

Despite significant improvements in life expectancy of kidney transplant patients due to advances in surgery and immunosuppression, Chronic Allograft Nephropathy (CAN) remains a daunting problem. A complex network of cellular mechanisms in both graft and peripheral immune compartments complicates the non-invasive diagnosis of CAN, which still requires biopsy histology. This is compounded by non-immunological factors contributing to graft injury. There is a pressing need to identify and validate minimally invasive biomarkers for CAN to serve as early predictors of graft loss and as metrics for managing long-term immunosuppression.

Methods

We used DNA microarrays, tandem mass spectroscopy proteomics and bioinformatics to identify genomic and proteomic markers of mil

SUBMITTER: Kurian SM 

PROVIDER: S-EPMC2703807 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature