Identification of diverse nerve growth factor-regulated genes by serial analysis of gene expression (SAGE) profiling.

ABSTRACT: Neurotrophic factors such as nerve growth factor (NGF) promote a wide variety of responses in neurons, including differentiation, survival, plasticity, and repair. Such actions often require changes in gene expression. To identify the regulated genes and thereby to more fully understand the NGF mechanism, we carried out serial analysis of gene expression (SAGE) profiling of transcripts derived from rat PC12 cells before and after NGF-promoted neuronal differentiation. Multiple criteria supported the reliability of the profile. Approximately 157,000 SAGE tags were analyzed, representing at least 21,000 unique transcripts. Of these, nearly 800 were regulated by 6-fold or more in response to NGF. Approximately 150 of the regulated transcripts have been matched to named genes, the majority of

SUBMITTER: Angelastro JM 

PROVIDER: S-EPMC27040 | biostudies-literature | 2000 Sep

REPOSITORIES: biostudies-literature