DNA Sudoku--harnessing high-throughput sequencing for multiplexed specimen analysis.

ABSTRACT: Next-generation sequencers have sufficient power to analyze simultaneously DNAs from many different specimens, a practice known as multiplexing. Such schemes rely on the ability to associate each sequence read with the specimen from which it was derived. The current practice of appending molecular barcodes prior to pooling is practical for parallel analysis of up to many dozen samples. Here, we report a strategy that permits simultaneous analysis of tens of thousands of specimens. Our approach relies on the use of combinatorial pooling strategies in which pools rather than individual specimens are assigned barcodes. Thus, the identity of each specimen is encoded within the pooling pattern rather than by its association with a particular sequence tag. Decoding the pattern allows the sequenc

SUBMITTER: Erlich Y 

PROVIDER: S-EPMC2704425 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature