Lack of bystander activation shows that localization exterior to chromosome territories is not sufficient to up-regulate gene expression.

ABSTRACT: Position within chromosome territories and localization at transcription factories are two facets of nuclear organization that have been associated with active gene expression. However, there is still debate about whether this organization is a cause or consequence of transcription. Here we induced looping out from chromosome territories (CTs), by the activation of Hox loci during differentiation, to investigate consequences on neighboring loci. We show that, even though flanking genes are caught up in the wave of nuclear reorganization, there is no effect on their expression. However, there is a differential organization of active and inactive alleles of these genes. Inactive alleles are preferentially retained within the CT, whereas actively transcribing alleles, and those associated wit

SUBMITTER: Morey C 

PROVIDER: S-EPMC2704431 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature