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Identification of a novel alkaliphilic esterase active at low temperatures by screening a metagenomic library from antarctic desert soil.


ABSTRACT: A novel esterase was identified through functional screening of a metagenomic library in Escherichia coli obtained from Antarctic desert soil. The 297-amino-acid sequence had only low (<29%) similarity to a putative esterase from Burkholderia xenovorans. The enzyme was active over a temperature range of 7 to 54 degrees C and at alkaline pH levels and is a potential candidate for industrial application.

SUBMITTER: Heath C 

PROVIDER: S-EPMC2704825 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature