Phylogeny and virulence of naturally occurring type III secretion system-deficient Pectobacterium strains.

ABSTRACT: Pectobacterium species are enterobacterial plant-pathogenic bacteria that cause soft rot disease in diverse plant species. Previous epidemiological studies of Pectobacterium species have suffered from an inability to identify most isolates to the species or subspecies level. We used three previously described DNA-based methods, 16S-23S intergenic transcribed spacer PCR-restriction fragment length polymorphism analysis, multilocus sequence analysis (MLSA), and pulsed-field gel electrophoresis, to examine isolates from diseased stems and tubers and found that MLSA provided the most reliable classification of isolates. We found that strains belonging to at least two Pectobacterium clades were present in each field examined, although representatives of only three of five Pectobacterium clades

SUBMITTER: Kim HS 

PROVIDER: S-EPMC2704834 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature