Mouse ACF7 and drosophila short stop modulate filopodia formation and microtubule organisation during neuronal growth.

ABSTRACT: Spectraplakins are large actin-microtubule linker molecules implicated in various processes, including gastrulation, wound healing, skin blistering and neuronal degeneration. Expression data for the mammalian spectraplakin ACF7 and genetic analyses of the Drosophila spectraplakin Short stop (Shot) suggest an important role during neurogenesis. Using three parallel neuronal culture systems we demonstrate that, like Shot, ACF7 is essential for axon extension and describe, for the first time, their subcellular functions during axonal growth. Firstly, both ACF7 and Shot regulate the organisation of neuronal microtubules, a role dependent on both the F-actin- and microtubule-binding domains. This role in microtubule organisation is probably the key mechanism underlying the roles of Shot and ACF

SUBMITTER: Sanchez-Soriano N 

PROVIDER: S-EPMC2704885 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature