Proliferation of L02 human hepatocytes in tolerized genetically immunocompetent rats.

ABSTRACT:

Aim

To investigate whether human hepatocytes could proliferate after transplantation to normal immunocompetent rats treated with 2-acetaminofluorene or retrorsine and partial hepatectomy.

Methods

L02 hepatocyte-tolerant Sprague-Dawley rats were injected with retrorsine, 2-acetaminofluorene or normal saline. L02 hepatocytes were then transplanted via the spleen. Human albumin and its mRNA, specific proliferating cell nuclear antigen (PCNA), L02 hepatocyte dynamic distribution, number density and area density of PCNA-positive cells in the liver were determined.

Results

All the examined indicators were not significantly different between the rats treated with 2-acetaminofluorene and normal saline, which was not the case with rats treated with retrorsine. A dynamic distri

SUBMITTER: Lin H 

PROVIDER: S-EPMC2705086 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature