Characterization of clarithromycin resistance in Malaysian isolates of Helicobacter pylori.

ABSTRACT:

Aim

To characterize the types of mutations present in the 23S rRNA genes of Malaysian isolates of clarithromycin-resistant Helicobacter pylori (H pylori).

Methods

Clarithromycin susceptibility of H pylori isolates was determined by E test. Analyses for point mutations in the domain V of 23S rRNA genes in clarithromycin-resistant and -sensitive strains were performed by sequence analysis of amplified polymerase chain reaction products. Restriction fragment length polymorphism was performed using BsaI and MboII enzymes to detect restriction sites that correspond to the mutations in the clarithromycin-resistant strains.

Results

Of 187 isolates from 120 patients, four were resistant to clarithromycin, while 183 were sensitive. The MIC of the resistant strains ranged from

SUBMITTER: Ahmad N 

PROVIDER: S-EPMC2705740 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature