Proteomic analysis of DNA-protein cross-linking by antitumor nitrogen mustards.

ABSTRACT: Nitrogen mustards are antitumor agents used clinically for the treatment of a variety of neoplastic conditions. The biological activity of these compounds is typically attributed to their ability to induce DNA-DNA cross-links. However, nitrogen mustards are able to produce a variety of other lesions, including DNA-protein cross-links (DPCs). DPCs induced by nitrogen mustards are not well-characterized because of their structural complexity and the insufficient specificity and sensitivity of previously available experimental methodologies. In the present work, affinity capture methodology in combination with mass spectrometry-based proteomics was employed to identify mammalian proteins that form covalent cross-links to DNA in the presence of a simple nitrogen mustard, mechlorethamine. Follo

SUBMITTER: Loeber RL 

PROVIDER: S-EPMC2706101 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature