Mustn1 is expressed during chondrogenesis and is necessary for chondrocyte proliferation and differentiation in vitro.

ABSTRACT: Mustn1 encodes a small nuclear protein expressed specifically in the musculoskeletal system that was originally identified as a strongly up-regulated gene during bone regeneration, especially in fracture callus proliferating chondrocytes. Further experiments were undertaken to investigate its expression and role during chondrogenesis. Initially, whole mount mouse in situ hybridization was carried out and revealed Mustn1 expression in areas of active chondrogenesis that included limb buds, branchial arches and tail bud. To elucidate its function, experiments were carried out to perturb Mustn1 by overexpression and silencing in the pre-chondrocytic RCJ3.1C5.18 (RCJ) cell line. In these cells, Mustn1 is normally differentially regulated, with a spike in expression 2 days after induction of di

SUBMITTER: Gersch RP 

PROVIDER: S-EPMC2706297 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature