A novel in vivo murine model of cartilage regeneration. Age and strain-dependent outcome after joint surface injury.

ABSTRACT:

Objectives

To generate and validate a murine model of joint surface repair following acute mechanical injury.

Methods

Full thickness defects were generated in the patellar groove of C57BL/6 and DBA/1 mice by microsurgery. Control knees were either sham-operated or non-operated. Outcome was evaluated by histological scoring systems. Apoptosis and proliferation were studied using TUNEL and Phospho-Histone H3 staining, respectively. Type II collagen neo-deposition and degradation were evaluated by immunostaining using antibodies to the CPII telopeptide and C1,2C (Col2-3/4Cshort), respectively. Aggrecanases and matrix metalloproteinases (MMPs) activity were assessed by immunostaining for TEGE(373) and VDIPEN neo-epitopes.

Results

Young 8-week-old DBA/1 mice displayed cons

SUBMITTER: Eltawil NM 

PROVIDER: S-EPMC2706394 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature