SIMS and MALDI MS imaging of the spinal cord.

ABSTRACT: The application of MS to imaging, or MS imaging (MSI), allows for the direct investigation of tissue sections to identify biological compounds and determine their spatial distribution. We present an approach to MSI that combines secondary ion MS (SIMS) and MALDI MS for the imaging and analysis of rat spinal cord sections, thereby enhancing the chemical coverage obtained from an MSI experiment. The spinal cord is organized into discrete, anatomically defined areas that include motor and sensory networks composed of chemically diverse cells. The MSI data presented here reveal the spatial distribution of multiple phospholipids, proteins, and neuropeptides obtained within single, 20 mum sections of rat spinal cord. Analyte identities are initially determined by primary mass match and confirmed

SUBMITTER: Monroe EB 

PROVIDER: S-EPMC2706659 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature