Klassevirus 1, a previously undescribed member of the family Picornaviridae, is globally widespread.

ABSTRACT:

Background

Diarrhea is the third leading infectious cause of death worldwide and is estimated to be responsible for approximately 2 million deaths a year. While many infectious causes of diarrhea have been established, approximately 40% of all diarrhea cases are of unknown etiology. In an effort to identify novel viruses that may be causal agents of diarrhea, we used high throughput mass sequencing to analyze stool samples collected from patients with acute diarrhea.

Results

Sequences with limited similarity to known picornaviruses were detected in a stool sample collected in Australia from a child with acute diarrhea. Using a combination of mass sequencing, RT-PCR, 5' RACE and 3' RACE, a 6383 bp fragment of the viral genome was sequenced. Phylogenetic analysis demonstrated

SUBMITTER: Holtz LR 

PROVIDER: S-EPMC2706813 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature