Tissue-specific variation in DNA methylation levels along human chromosome 1.

ABSTRACT:

Background

DNA methylation is a major epigenetic modification important for regulating gene expression and suppressing spurious transcription. Most methods to scan the genome in different tissues for differentially methylated sites have focused on the methylation of CpGs in CpG islands, which are concentrations of CpGs often associated with gene promoters.

Results

Here, we use a methylation profiling strategy that is predominantly responsive to methylation differences outside of CpG islands. The method compares the yield from two samples of size-selected fragments generated by a methylation-sensitive restriction enzyme. We then profile nine different normal tissues from two human donors relative to spleen using a custom array of genomic clones covering the euchromatic portio

SUBMITTER: De Bustos C 

PROVIDER: S-EPMC2706828 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature