Expression, purification and in vitro functional reconstitution of the chemokine receptor CCR1.

ABSTRACT: Chemokine receptors are a specific class of G-protein-coupled receptors (GPCRs) that control cell migration associated with routine immune surveillance, inflammation and development. In addition to their roles in normal physiology, these receptors and their ligands are involved in a large number of inflammatory diseases, cancer and AIDS, making them prime therapeutic targets in the pharmaceutical industry. Like other GPCRs, a significant obstacle in determining structures and characterizing mechanisms of activation has been the difficulty in obtaining high levels of pure, functional receptor. Here we describe a systematic effort to express the chemokine receptor CCR1 in mammalian cells, and to purify and reconstitute it in functional form. The highest expression levels were obtained using

SUBMITTER: Allen SJ 

PROVIDER: S-EPMC2706832 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature