Implementation of a data repository-driven approach for targeted proteomics experiments by multiple reaction monitoring.

ABSTRACT: Multiple reaction monitoring (MRM), commonly employed for the mass spectrometric detection of small molecules, is rapidly gaining ground in proteomics. Its high sensitivity and specificity makes this targeted approach particularly useful when sample throughput or proteome coverage limits global studies. Existing tools to design MRM assays rely exclusively on theoretical predictions, or combine them with previous observations on the same type of sample. The additional mass spectrometric experimentation this requires can pose significant demands on time and material. To overcome these challenges, a new MRM worksheet was introduced into The Global Proteome Machine database (GPMDB) that provided all of the information needed to design MRM transitions based solely on archived observations made

SUBMITTER: Walsh GM 

PROVIDER: S-EPMC2706936 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature