Role of 20-kDa amelogenin (P148) phosphorylation in calcium phosphate formation in vitro.

ABSTRACT: The potential role of amelogenin phosphorylation in enamel formation is elucidated through in vitro mineralization studies. Studies focused on the native 20-kDa porcine amelogenin proteolytic cleavage product P148 that is prominent in developing enamel. Experimental conditions supported spontaneous calcium phosphate precipitation with the initial formation of amorphous calcium phosphate (ACP). In the absence of protein, ACP was found to undergo relatively rapid transformation to randomly oriented plate-like apatitic crystals. In the presence of non-phosphorylated recombinant full-length amelogenin, rP172, a longer induction period was observed during which relatively small ACP nanoparticles were transiently stabilized. In the presence of rP172, these nanoparticles were found to align to fo

SUBMITTER: Kwak SY 

PROVIDER: S-EPMC2707234 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature