Thiourea modulates the expression and activity profile of mtATPase under salinity stress in seeds of Brassica juncea.

ABSTRACT:

Background and aims

Large areas of the globe are becoming saline due to evapotranspiration and poor irrigation practices, and sustainability of agriculture is being seriously affected. Thiourea (TU) has been identified as an effective bioregulator imparting stress tolerance to crops. The molecular mechanisms involved in the TU-mediated response are considered in this study.

Methods

Differential display was performed in order to identify TU-modulated transcripts in Brassica juncea seeds exposed to various treatments (distilled water; 1 m NaCl; 1 m NaCl + 500 p.p.m. TU). The differential regulation of these transcripts was validated by quantitative real-time PCR.

Key results

Thiourea treatment maintained the viability of seeds exposed to NaCl for 6 h. Expression analysi

SUBMITTER: Srivastava AK 

PROVIDER: S-EPMC2707324 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature