Vault nanoparticles containing an adenovirus-derived membrane lytic protein facilitate toxin and gene transfer.

ABSTRACT: Nonviral methods of gene delivery possess several advantages over that of viral-based vectors, including having increased safety. However, the ability to achieve effective transport of therapeutic molecules across host cell membranes via nonviral methods remains a significant goal. Cell-derived nanoparticles known as vaults have been proposed as novel candidate transfer vehicles for various foreign molecules. Recombinant vault particles enter cells via macropinocytosis or phagocytosis but lack demonstrable membrane penetrating activity. To explore the feasibility of improving vault penetration into target cells, we incorporated the membrane lytic domain of adenovirus protein VI (pVI) into the interior of recombinant vault particles via fusion to the vault poly(ADP-ribose) polymerase (VPARP

SUBMITTER: Lai CY 

PROVIDER: S-EPMC2707358 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature