Protease activated receptor signaling is required for African trypanosome traversal of human brain microvascular endothelial cells.

ABSTRACT:

Background

Using human brain microvascular endothelial cells (HBMECs) as an in vitro model for how African trypanosomes cross the human blood-brain barrier (BBB) we recently reported that the parasites cross the BBB by generating calcium activation signals in HBMECs through the activity of parasite cysteine proteases, particularly cathepsin L (brucipain). In the current study, we examined the possible role of a class of protease stimulated HBMEC G protein coupled receptors (GPCRs) known as protease activated receptors (PARs) that might be implicated in calcium signaling by African trypanosomes.

Methodology/principal findings

Using RNA interference (RNAi) we found that in vitro PAR-2 gene (F2RL1) expression in HBMEC monolayers could be reduced by over 95%. We also found that

SUBMITTER: Grab DJ 

PROVIDER: S-EPMC2707606 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature