Proteomic identification of small, copper-responsive proteins in germinating embryos of Oryza sativa.

ABSTRACT:

Background and aims

Although copper (Cu) is an essential micronutrient for plants and algae, excess Cu is toxic to most plants and can cause a wide range of deleterious effects. To investigate the response of rice (Oryza sativa) to Cu stress, a proteomic approach was used to analyse Cu stress-induced changes in the expression of low molecular-weight proteins in germinating rice seed embryos.

Methods

Rice seeds were germinated in the presence or absence of 200 microm Cu for 6 d, and embryos, including newly formed shoots and radicles, were isolated. After proteins were extracted from the germinating embryos and separated by two-dimensional PAGE, 16 proteins in the 6- to 25-kDa range were identified using MALDI-TOF mass spectrometry.

Key results and conclusions

Thirteen

SUBMITTER: Zhang H 

PROVIDER: S-EPMC2707895 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature