Mechanisms of U46619-induced contraction of rat pulmonary arteries in the presence and absence of the endothelium.

ABSTRACT:

Background and purpose

Thromboxane A(2) and endothelial dysfunction are implicated in the development of pulmonary hypertension. The receptor-transduction pathway for U46619 (9,11-dideoxy-9 alpha, 11 alpha-methanoepoxy prostaglandin F(2 alpha))-induced contraction was examined in endothelium-intact (E+) and denuded (E-) rat pulmonary artery rings.

Experimental approach

Artery rings were mounted on a wire myograph under a tension of 7-7.5 mN at 37 degrees C and gassed with 95% O(2)/5% CO(2). Isometric recording was made by using Powerlab data collection and Chart 5 software.

Key results

Both E+ and E- contractile responses were sensitive to Rho-kinase inhibition and the chloride channel blocker NPPB [5-nitro-2-(3-phenylpropylamino)benzoic acid]. The E+ response was sen

SUBMITTER: McKenzie C 

PROVIDER: S-EPMC2707971 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature