The role of the PDE4D cAMP phosphodiesterase in the regulation of glucagon-like peptide-1 release.

ABSTRACT:

Background and purpose

Increases in intracellular cyclic AMP (cAMP) augment the release/secretion of glucagon-like peptide-1 (GLP-1). As cAMP is hydrolysed by cAMP phosphodiesterases (PDEs), we determined the role of PDEs and particularly PDE4 in regulating GLP-1 release.

Experimental approach

GLP-1 release, PDE expression and activity were investigated using rats and GLUTag cells, a GLP-1-releasing cell line. The effects of rolipram, a selective PDE4 inhibitor both in vivo and in vitro and stably overexpressed catalytically inactive PDE4D5 (D556A-PDE4D5) mutant in vitro on GLP-1 release were investigated.

Key results

Rolipram (1.5 mg x kg(-1) i.v.) increased plasma GLP-1 concentrations approximately twofold above controls in anaesthetized rats and enhanced glucose-in

SUBMITTER: Ong WK 

PROVIDER: S-EPMC2707975 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature