Denaturing gradient gel electrophoresis of PCR-amplified gki genes: a new technique for tracking streptococci.

ABSTRACT: Viridans group streptococci (VGS) are a well-known cause of infections in immunocompromised patients, accounting for severe morbidity and mortality. Streptococcus mitis group species (Streptococcus mitis, Streptococcus pneumoniae, Streptococcus oralis) are among the VGS most often encountered in clinical practice. Identifying the portal of entry for S. mitis group strains is crucial for interventions preventing bacterial translocation. Unfortunately, tracking the source of S. mitis group strains is dependent on a combination of extremely laborious and time-consuming cultivation and molecular techniques (enterobacterial repetitive intergenic consensus-PCR [ERIC-PCR]). To simplify this procedure, a PCR analysis with newly designed primers targeting the household gene glucose kinase (gki) was

SUBMITTER: van Vliet MJ 

PROVIDER: S-EPMC2708528 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature