Proteomics-based identification of anchorless cell wall proteins as vaccine candidates against Staphylococcus aureus.

ABSTRACT: Staphylococcus aureus is an important human pathogen with increasing clinical impact due to the extensive spread of antibiotic-resistant strains. Therefore, development of a protective polyvalent vaccine is of great clinical interest. We employed an intravenous immunoglobulin (IVIG) preparation as a source of antibodies directed against anchorless S. aureus surface proteins for identification of novel vaccine candidates. In order to identify such proteins, subtractive proteome analysis (SUPRA) of S. aureus anchorless cell wall proteins was performed. Proteins reacting with IVIG but not with IVIG depleted of S. aureus-specific opsonizing antibodies were considered vaccine candidates. Nearly 40 proteins were identified by this preselection method using matrix-assisted laser desorption ioniza

SUBMITTER: Glowalla E 

PROVIDER: S-EPMC2708538 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature