Paradoxical effects of PDGF-BB overexpression in endothelial cells on engineered blood vessels in vivo.

ABSTRACT: Therapeutic revascularization with either exogenous angiogenic growth factors or vascular cells has yet to demonstrate efficacy in the clinic. Injection of angiogenic growth factors often produces unstable and abnormal blood vessels. Blood vascular networks derived from implanted endothelial cells persist only transiently due to the insufficient recruitment of perivascular cells. We hypothesize that a combination of the two approaches may act synergistically to yield a better result. To enhance the recruitment of perivascular cells, human umbilical vein endothelial cells were genetically modified to overexpress platelet-derived growth factor (PDGF)-BB. PDGF-BB overexpression promoted both proliferation and migration of perivascular precursor cells (10T1/2 cells) in vitro. When mock-infecte

SUBMITTER: Au P 

PROVIDER: S-EPMC2708815 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature