Progesterone activates a progesterone receptor membrane component 1-dependent mechanism that promotes human granulosa/luteal cell survival but not progesterone secretion.

ABSTRACT:

Context

Progesterone (P4) promotes its own secretion and the survival of human granulosa/luteal (GL) cells.

Objective

The objective of these studies was to determine whether progesterone receptor membrane component-1 (PGRMC1) mediates P4's actions.

Design

In vitro studies were conducted on GL cells from women undergoing in vitro fertilization and GL5 cells, which are derived from GL cells.

Setting and patients

GL cells were obtained from women undergoing fertility treatment at a university-based clinic and used for in vitro studies.

Main outcome measures

PCR, Western blot, and immunocytochemistry were used to detect various progestin binding proteins. (3)H-P4 binding kinetics were assessed on partially purified PGRMC1. Apoptosis was determined after cult

SUBMITTER: Peluso JJ 

PROVIDER: S-EPMC2708946 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature