A proapoptotic signaling pathway involving RasGRP, Erk, and Bim in B cells.

ABSTRACT:

Objective

Bryostatin-1 and related diacylglycerol (DAG) analogues activate RasGRPs in lymphocytes, thereby activating Ras and mimicking some aspects of immune receptor signaling. To define the role of RasGRPs in lymphocyte apoptosis and to identify potential therapeutic uses for DAG analogues in lymphocyte disorders, we characterized the response of lymphoma-derived cell lines to DAG analogues.

Materials and methods

Human lymphoma-derived B cell lines and mouse primary B cells were treated with bryostatin-1 or its synthetic analogue "pico." Ras signaling partners and Bcl-2 family members were studied with biochemical assays. Cellular responses were monitored using growth and apoptosis assays.

Results

Stimulation of B cells with DAG analogues results in activation of p

SUBMITTER: Stang SL 

PROVIDER: S-EPMC2708980 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature