Cooperative effects on radical recombination in CYP3A4-catalyzed oxidation of the radical clock beta-thujone.

ABSTRACT: The timing of the beta-thujone radical clock (see scheme) can be specifically altered by an allosteric effector. Progesterone, a well-documented CYP3A4 allosteric effector, was found to increase the yield of the unrearranged, C4-derived product of beta-thujone oxidation at the expense of the combined yields of all the rearranged C4-oxidized metabolites. The results demonstrate that the apparent radical recombination rate in the CYP3A4 hydroxylation of beta-thujone is accelerated by the progesterone heterotropic cooperativity.

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC2709176 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature