Genome-wide transcription analysis of histidine-related cataract in Atlantic salmon (Salmo salar L).

ABSTRACT:

Purpose

Elevated levels of dietary histidine have previously been shown to prevent or mitigate cataract formation in farmed Atlantic salmon (Salmo salar L). The aim of this study was to shed light on the mechanisms by which histidine acts. Applying microarray analysis to the lens transcriptome, we screened for differentially expressed genes in search for a model explaining cataract development in Atlantic salmon and possible markers for early cataract diagnosis.

Methods

Adult Atlantic salmon (1.7 kg) were fed three standard commercial salmon diets only differing in the histidine content (9, 13, and 17 g histidine/kg diet) for four months. Individual cataract scores for both eyes were assessed by slit-lamp biomicroscopy. Lens N-acetyl histidine contents were measured by high

SUBMITTER: Trosse C 

PROVIDER: S-EPMC2709424 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature