Ontology highlight
ABSTRACT:
SUBMITTER: van Loenhout MT
PROVIDER: S-EPMC2709578 | biostudies-literature | 2009 Jul
REPOSITORIES: biostudies-literature
Nucleic acids research 20090508 12
RecA, the key protein in homologous recombination, performs its actions as a helical filament on single-stranded DNA (ssDNA). ATP hydrolysis makes the RecA-ssDNA filament dynamic and is essential for successful recombination. RecA has been studied extensively by single-molecule techniques on double-stranded DNA (dsDNA). Here we directly probe the structure and kinetics of RecA interaction with its biologically most relevant substrate, long ssDNA molecules. We find that RecA ATPase activity is re ...[more]