Using high-density exon arrays to profile gene expression in closely related species.

ABSTRACT: Global comparisons of gene expression profiles between species provide significant insight into gene regulation, evolutionary processes and disease mechanisms. In this work, we describe a flexible and intuitive approach for global expression profiling of closely related species, using high-density exon arrays designed for a single reference genome. The high-density probe coverage of exon arrays allows us to select identical sets of perfect-match probes to measure expression levels of orthologous genes. This eliminates a serious confounding factor in probe affinity effects of species-specific microarray probes, and enables direct comparisons of estimated expression indexes across species. Using a newly designed Affymetrix exon array, with eight probes per exon for approximately 315,000 exon

SUBMITTER: Lin L 

PROVIDER: S-EPMC2709591 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature