Characterization and isolation of a T-DNA tagged banana promoter active during in vitro culture and low temperature stress.

ABSTRACT:

Background

Next-generation transgenic plants will require a more precise regulation of transgene expression, preferably under the control of native promoters. A genome-wide T-DNA tagging strategy was therefore performed for the identification and characterization of novel banana promoters. Embryogenic cell suspensions of a plantain-type banana were transformed with a promoterless, codon-optimized luciferase (luc+) gene and low temperature-responsive luciferase activation was monitored in real time.

Results

Around 16,000 transgenic cell colonies were screened for baseline luciferase activity at room temperature 2 months after transformation. After discarding positive colonies, cultures were re-screened in real-time at 26 degrees C followed by a gradual decrease to 8 degrees C

SUBMITTER: Santos E 

PROVIDER: S-EPMC2709630 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature