A BAC-based physical map of the Hessian fly genome anchored to polytene chromosomes.

ABSTRACT:

Background

The Hessian fly (Mayetiola destructor) is an important insect pest of wheat. It has tractable genetics, polytene chromosomes, and a small genome (158 Mb). Investigation of the Hessian fly presents excellent opportunities to study plant-insect interactions and the molecular mechanisms underlying genome imprinting and chromosome elimination. A physical map is needed to improve the ability to perform both positional cloning and comparative genomic analyses with the fully sequenced genomes of other dipteran species.

Results

An FPC-based genome wide physical map of the Hessian fly was constructed and anchored to the insect's polytene chromosomes. Bacterial artificial chromosome (BAC) clones corresponding to 12-fold coverage of the Hessian fly genome were fingerprinted,

SUBMITTER: Aggarwal R 

PROVIDER: S-EPMC2709663 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature