Expression and alternative splicing of folate pathway genes in HapMap lymphoblastoid cell lines.

ABSTRACT:

Aim

Folate is vital for cell growth and development through its important role in one-carbon metabolism - an essential process in the synthesis of amino acids and nucleic acids. Folate pathway genes have been considered as therapeutic targets of drugs for the treatment of cancer and other diseases. Racial and ethnic disparities of folate metabolism and outcome of antifolate therapies have been reported. In this study, we evaluate the genetic regulation for expression and alternative splicing of folate related genes in HapMap lymphoblastoid cell lines (LCLs) of individuals of European and African descent.

Materials & methods

Gene and exon level expression and alternative splicing of folate pathway genes were compared in LCLs derived from the Centre d'Etude du Polymorphisme Hu

SUBMITTER: Duan S 

PROVIDER: S-EPMC2709842 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature