NMD inhibition fails to identify tumour suppressor genes in microsatellite stable gastric cancer cell lines.

ABSTRACT:

Background

Gastric cancers frequently show chromosomal alterations which can cause activation of oncogenes, and/or inactivation of tumour suppressor genes. In gastric cancer several chromosomal regions are described to be frequently lost, but for most of the regions, no tumour suppressor genes have been identified yet. The present study aimed to identify tumour suppressor genes inactivated by nonsense mutation and deletion in gastric cancer by means of GINI (gene identification by nonsense mediated decay inhibition) and whole genome copy number analysis.

Methods

Two non-commercial gastric cancer cell lines, GP202 and IPA220, were transfected with siRNA directed against UPF1, to specifically inhibit the nonsense mediated decay (NMD) pathway, and with siRNA directed against no

SUBMITTER: Buffart TE 

PROVIDER: S-EPMC2709900 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature