Genomic origin and organization of the allopolyploid Primula egaliksensis investigated by in situ hybridization.

ABSTRACT:

Background and aims

Earlier studies have suggested that the tetraploid Primula egaliksensis (2n = 40) originated from hybridization between the diploids P. mistassinica (2n = 18) and P. nutans (2n = 22), which were hypothesized to be the maternal and paternal parent, respectively. The present paper is aimed at verifying the hybrid nature of P. egaliksensis using cytogenetic tools, and to investigate the extent to which the parental genomes have undergone genomic reorganization.

Methods

Genomic in situ hybridization (GISH) and fluorescent in situ hybridization (FISH) with ribosomal DNA (rDNA) probes, together with sequencing of the internal transcribed spacer (ITS) region of the rDNA, were used to identify the origin of P. egaliksensis and to explore its genomic organization,

SUBMITTER: Guggisberg A 

PROVIDER: S-EPMC2710232 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature