The novel RASSF6 and RASSF10 candidate tumour suppressor genes are frequently epigenetically inactivated in childhood leukaemias.

ABSTRACT:

Background

The Ras-association family (RASSF) of tumour suppressor genes (TSGs) contains 10 members that encode proteins containing Ras-association (RA) domains. Several members of the RASSF family are frequently epigenetically inactivated in cancer, however, their role in leukaemia has remained largely uninvestigated. Also, RASSF10 is a predicted gene yet to be experimentally verified. Here we cloned, characterised and demonstrated expression of RASSF10 in normal human bone marrow. We also determined the methylation status of CpG islands associated with RASSF1-10 in a series of childhood acute lymphocytic leukaemias (ALL) and normal blood and bone marrow samples.

Results

COBRA and bisulphite sequencing revealed RASSF6 and RASSF10 were the only RASSF members with a high freq

SUBMITTER: Hesson LB 

PROVIDER: S-EPMC2711046 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature