Identification of a novel nucleolin related protein (NRP) gene expressed during rat spermatogenesis.

ABSTRACT:

Background

Nucleolin is a major nucleolar phosphoprotein involved in various steps of ribosome biogenesis in eukaryotic cells. As nucleolin plays a significant role in ribosomal RNA transcription we were interested in examining in detail the expression of nucleolin across different stages of spermatogenesis and correlate with the transcription status of ribosomal DNA in germ cells.

Results

By RT PCR and western blot analysis we found that nucleolin is strongly down regulated in meiotic spermatocytes and haploid germ cells. We have identified a new nucleolin related protein (NRP) gene in the rat genome, which is over expressed in the testis and is up regulated several fold in meiotic spermatocytes and haploid germ cells. The NRP protein lacks the acidic stretches in its N ter

SUBMITTER: Chathoth KT 

PROVIDER: S-EPMC2711064 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature