Impact of animal strain on gene expression in a rat model of acute cardiac rejection.

ABSTRACT:

Background

The expression levels of many genes show wide natural variation among strains or populations. This study investigated the potential for animal strain-related genotypic differences to confound gene expression profiles in acute cellular rejection (ACR). Using a rat heart transplant model and 2 different rat strains (Dark Agouti, and Brown Norway), microarrays were performed on native hearts, transplanted hearts, and peripheral blood mononuclear cells (PBMC).

Results

In heart tissue, strain alone affected the expression of only 33 probesets while rejection affected the expression of 1368 probesets (FDR 10% and FC > o= 3). Only 13 genes were affected by both strain and rejection, which was < 1% (13/1368) of all probesets differentially expressed in ACR. However, for P

SUBMITTER: Deans KJ 

PROVIDER: S-EPMC2711118 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature