Substrate topography induces a crossover from 2D to 3D behavior in fibroblast migration.

ABSTRACT: In a three-dimensional environment, cells migrate through complex topographical features. Using microstructured substrates, we investigate the role of substrate topography in cell adhesion and migration. To do so, fibroblasts are plated on chemically identical substrates composed of microfabricated pillars. When the dimensions of the pillars (i.e., the diameter, length, and spacing) are varied, migrating cells encounter alternating flat and rough surfaces that depend on the spacing between the pillars. Consequently, we show that substrate topography affects cell shape and migration by modifying cell-to-substrate interactions. Cells on micropillar substrates exhibit more elongated and branched shapes with fewer actin stress fibers compared with cells on flat surfaces. By analyzing the migra

SUBMITTER: Ghibaudo M 

PROVIDER: S-EPMC2711370 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature