Patterns of receptor revision in the immunoglobulin heavy chains of a teleost fish.

ABSTRACT: H chain cDNA libraries were constructed from the RNA derived from seven different organs and tissues from the same individual catfish. Sequence analysis of >300 randomly selected clones identified clonal set members within the same or different tissues, and some of these represented mosaic or hybrid sequences. These hybrids expressed V(H) members of the same or different V(H) families within different regions of the same clone. Within some clonal sets multiple hybrids were identified, and some of these represented the products of sequential V(H) replacement events. Different experimental methods confirmed that hybrid clones identified in the cDNA library from one tissue could be reisolated in the cDNA pool or from the total RNA derived from the same or a different tissue, indicating that t

SUBMITTER: Lange MD 

PROVIDER: S-EPMC2711631 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature