Production and characterisation of monoclonal antibodies against RAI3 and its expression in human breast cancer.

ABSTRACT:

Background

RAI3 is an orphan G-protein coupled receptor (GPCR) that has been associated with malignancy and may play a role in the proliferation of breast cancer cells. Although its exact function in normal and malignant cells remains unclear and evidence supporting its role in oncogenesis is controversial, its abundant expression on the surface of cancer cells would make it an interesting target for the development of antibody-based therapeutics. To investigate the link with cancer and provide more evidence for its role, we carried out a systematic analysis of RAI3 expression in a large set of human breast cancer specimens.

Methods

We expressed recombinant human RAI3 in bacteria and reconstituted the purified protein in liposomes to raise monoclonal antibodies using classic

SUBMITTER: Jorissen H 

PROVIDER: S-EPMC2711971 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature