Strong correlation between SHAPE chemistry and the generalized NMR order parameter (S2) in RNA.

ABSTRACT: The functions of most RNA molecules are critically dependent on the distinct local dynamics that characterize secondary structure and tertiary interactions and on structural changes that occur upon binding by proteins and small molecule ligands. Measurements of RNA dynamics at nucleotide resolution set the foundation for understanding the roles of individual residues in folding, catalysis, and ligand recognition. In favorable cases, local order in small RNAs can be quantitatively analyzed by NMR in terms of a generalized order parameter, S2. Alternatively, SHAPE (selective 2'-hydroxyl acylation analyzed by primer extension) chemistry measures local nucleotide flexibility in RNAs of any size using structure-sensitive reagents that acylate the 2'-hydroxyl position. In this work, we compare p

SUBMITTER: Gherghe CM 

PROVIDER: S-EPMC2712629 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature