Stabilization of dicentric translocations through secondary rearrangements mediated by multiple mechanisms in S. cerevisiae.

ABSTRACT:

Background

The gross chromosomal rearrangements (GCRs) observed in S. cerevisiae mutants with increased rates of accumulating GCRs include predicted dicentric GCRs such as translocations, chromosome fusions and isoduplications. These GCRs resemble the genome rearrangements found as mutations underlying inherited diseases as well as in the karyotypes of many cancers exhibiting ongoing genome instability

Methodology/principal findings

The structures of predicted dicentric GCRs were analyzed using multiple strategies including array-comparative genomic hybridization, pulse field gel electrophoresis, PCR amplification of predicted breakpoints and sequencing. The dicentric GCRs were found to be unstable and to have undergone secondary rearrangements to produce stable monocentric

SUBMITTER: Pennaneach V 

PROVIDER: S-EPMC2712687 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature