Selection of anti-sulfadimidine specific ScFvs from a hybridoma cell by eukaryotic ribosome display.

ABSTRACT:

Background

Ribosome display technology has provided an alternative platform technology for the development of novel low-cost antibody based on evaluating antibiotics derived residues in food matrixes.

Methodology/principal findings

In our current studies, the single chain variable fragments (scFvs) were selected from hybridoma cell lines against sulfadimidine (SM(2)) by using a ribosome library technology. A DNA library of scFv antibody fragments was constructed for ribosome display, and then mRNA-ribosome-antibody (MRA) complexes were produced by a rabbit reticulocyte lysate system. The synthetic sulfadimidine-ovalbumin (SM(2)-OVA) was used as an antigen to pan MRA complexes and putative scFv-encoding genes were recovered by RT-PCR in situ following each panning. After four

SUBMITTER: Qi Y 

PROVIDER: S-EPMC2712767 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature