Biological basis for restriction of microRNA targets to the 3' untranslated region in mammalian mRNAs.

ABSTRACT: MicroRNAs (miRNAs) interact with target sites located in the 3' untranslated regions (3' UTRs) of mRNAs to downregulate their expression when the appropriate miRNA is bound to target mRNA. To establish the functional importance of target-site localization in the 3' UTR, we modified the stop codon to extend the coding region of the transgene reporter through the miRNA target sequence. As a result, the miRNAs lost their ability to inhibit translation but retained their ability to function as small interfering RNAs in mammalian cells in culture and in vivo. The addition of rare but not optimal codons upstream of the extended opening reading frame (ORF) made the miRNA target site more accessible and restored miRNA-induced translational knockdown. Taken together, these results suggest that acti

SUBMITTER: Gu S 

PROVIDER: S-EPMC2713750 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature