Repertoire of bovine miRNA and miRNA-like small regulatory RNAs expressed upon viral infection.

ABSTRACT: MicroRNA (miRNA) and other types of small regulatory RNAs play a crucial role in the regulation of gene expression in eukaryotes. Several distinct classes of small regulatory RNAs have been discovered in recent years. To extend the repertoire of small RNAs characterized in mammals and to examine relationship between host miRNA expression and viral infection we used Illumina's ultrahigh throughput sequencing approach. We sequenced three small RNA libraries prepared from cell line derived from the adult bovine kidney under normal conditions and upon infection of the cell line with Bovine herpesvirus 1. We used a bioinformatics approach to distinguish authentic mature miRNA sequences from other classes of small RNAs and short RNA fragments represented in the sequencing data. Using this approa

SUBMITTER: Glazov EA 

PROVIDER: S-EPMC2713767 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature